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. Author manuscript; available in PMC: 2006 Jan 25.
Published in final edited form as: J Biol Chem. 1995 Jun 23;270(25):14983–14988. doi: 10.1074/jbc.270.25.14983

Fig. 4.

Fig. 4

CTP synthetase activities and the synthesis of CTP in S. cerevisiae expressing human CTP synthetase 1 and human CTP synthetase 2. The ura7Δ ura8Δ mutant bearing plasmids with the yeast URA7 gene (strain GHY52) or the human CTPS1 (strain GHY55) or CTPS2 (strain GHY56) genes were grown to the exponential phase of growth. Panel A, The cytosolic fraction was isolated from the cells and assayed for CTP synthetase activity from the indicated yeast (yCTPS) or human CTP synthetase 1 (hCTPS1) and CTP synthetase 2 (hCTPS2) enzymes. Panel B, CTP was extracted from the cells and analyzed by high performance liquid chromatography. Each data point represents the average of duplicate determinations from two independent experiments ± S.D.