Skip to main content
. Author manuscript; available in PMC: 2006 Jan 25.
Published in final edited form as: J Biol Chem. 1995 Jun 23;270(25):14983–14988. doi: 10.1074/jbc.270.25.14983

TABLE I.

Strains used in this work

Strain Relevant characteristics Source or Ref
E. coli
 DH5α F-, ϕ80dlacZΔM15, Δ(lacZYA-argF)U169, deoR, recA1, endA1, hsdR17(rk- mk+), phoA, supE44, λ-thi-1, gyrA96, relA1 (52)
 BL21(DE3) F- ompT hsdSB (rB-mB-) gal dcm (DE3) Novagen
S. cerevisiae
 SDO195 MATa leu2-3, 112 trp1-289 ura3-52 ura7Δ::TRP1 ura8Δ::hisG [pDO134] (9)
 GHY52 MATa leu2-3, 112 trp1-289 ura3-52 ura7Δ::TRP1 ura8Δ::hisG [pDO134] [pDO105] This study
 GHY53 MATa leu2-3, 112 trp1-289 ura3-52 ura7Δ::TRP1 ura8Δ::hisG [pDO134] [pDO105-hCTPS1] This study
 GHY54 MATa leu2-3, 112 trp1-289 ura3-52 ura7Δ::TRP1 ura8Δ::hisG [pDO134] [pDO105-hCTPS2] This study
 GHY55 MATa leu2-3, 112 trp1-289 ura3-52 ura7Δ::TRP1 ura8Δ::hisG [pDO105-hCTPS1] This study
 GHY56 MATa leu2-3, 112 trp1-289 ura3-52 ura7Δ::TRP1 ura8Δ::hisG [pDO105-hCTPS2] This study