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. Author manuscript; available in PMC: 2006 Jan 26.
Published in final edited form as: Mol Cell Proteomics. 2005 May 18;4(8):1061–1071. doi: 10.1074/mcp.M400207-MCP200

Fig. 2.

Fig. 2

Crosslinked CD147 complexes contain CD98hc. A) Transfected MCF7 cells were treated with no cross-linker (lanes 1, 4), DSP (lanes 2, 5) or BS3 (lanes 3, 6), and lysed using 1% Triton X-100. CD147 (lanes 1-3) and control SDFR1 (lanes 4–6) were immunoprecipitated using anti-FLAG antibody, and samples were blotted for endogenous CD98hc using pAb C-20. B) Transfected HT1080 cells were treated with or without crosslinker (as in Part A), CD147-FLAG, or negative control (SDFR1-FLAG) was immunoprecipitated, and samples were blotted for endogenous CD98hc. C) Transfected HEK293 cells were treated with or without crosslinker (as indicated), CD147-FLAG or control SDFR1-FLAG was immunoprecipitated, and samples were blotted blot for endogenous CD98hc. For cells used in panels A–C, CD147-FLAG and SDFR1-FLAG were expressed a comparable levels, as indicated by anti-FLAG blotting (not shown). D) Intact HEK293 cells were crosslinked (1 mM BS3), lysed in 1% Triton X-100, and then CD147-FLAG was immunoprecipitated and samples were blotted for MCT1. E) HEK293 cells were lysed in 1% Brij 97, CD147-FLAG was immunoprecipitated, and samples were blotted for MCT1.