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. 2002 Jul;184(13):3569–3577. doi: 10.1128/JB.184.13.3569-3577.2002

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Copyright © 2002, American Society for Microbiology

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FIG. 5. — Metal accumulation of E. coli CC118 producing HoxN variants N31H and N31I (grey symbols) and NhlF variants H34N and H34I (black symbols) during growth in Luria-Bertani medium. TMD I of each of the two permeases is shown in the upper part; the approximate locations of the HoxN residue N31 and the NhlF residue H34 are indicated. Transporters were produced from plasmids pCH675A (squares), pHoxNN31H (triangles), and pHoxNN31I (circles) for HoxN and from plasmids pCH674A (squares), pNhlFH34N (triangles), and pNhlFH34I (circles) for NhlF.