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Relative amounts of HoxN and NhlF variants in membranes of E. coli CC118. The mutant alleles were introduced into pCH675AF and pCH674AF, respectively, and expressed in strain CC118. Solubilized membrane proteins were separated by SDS-PAGE and blotted onto a nitrocellulose membrane. Transporters were visualized by using monoclonal anti-FLAG antibodies and alkaline phosphatase-coupled goat anti-mouse antibodies.
