Representative reverse transcriptase-polymerase chain reaction (RT-PCR) analyses of 17α-hydroxylase (CYP17α), endothelial (eNOS), neuronal (nNOS), and inducible (iNOS) messenger RNA (mRNA) in Leydig cells (ALC), testicular macrophages (TMs), and cerebellum (brain) of the rat. (A) Testicular macrophages were stimulated for 24 hours with lipopolysaccharide (LPS) (0.1 μg/mL) and then processed as described in “Materials and Methods.” (B) Samples of cerebellum and Leydig cells were examined to detect the presence of the specific mRNAs. Molecular markers are depicted under M. These experiments were repeated 4 times on separate samples with similar results.