Table 1.
Determination of l-[14C]citrulline formation in rat Leydig cells, cerebellum, and skeletal muscles*†
| Tissue | Particulate Fraction (fmol/min/mg of Protein/min) | Cytosol (fmol/min/mg of Protein/min) |
|---|---|---|
| Leydig cells | 413.7 ± 61.6 (7) | 167.4 ± 48.9 (6) |
| Leydig cells + EGTA | 515.2 ± 140.5 (3) | ND |
| Leydig cells + L-NAME | 550.4 ± 115.6 (3) | ND |
| Cerebellum | ND | 142 430 ± 19 450 (5) |
| Cerebellum + L-NAME | ND | 1427 ± 495.1 (3) |
| Cerebellum + EGTA | ND | 5843 ± 232.1 (3) |
| Skeletal muscle | 3828 ± 934.9 (6) | 441.5 ± 158.1 (6) |
*
Rat Leydig cells and muscle samples were prepared as described in “Materials and Methods.” Rat cerebellum extracts were from Stratagene. l-[14C]citrulline formation was estimated in duplicates using the Stratagene kit and is expressed as fmol/min/mg protein/min (mean ± SEM) with the number of experiments in parentheses. EGTA and L-NAME were added at a final concentration of 5 and 1 mM, respectively.
†
ND indicates not determined; EGTA, ethylenebis (oxyethylenenitrilo)tetraacetic acid; and L-NAME, L-NQ-nitro-arginine methyl ester.