. Author manuscript; available in PMC: 2006 Jan 26.

Published in final edited form as: J Androl. 2005;26(3):369–378. doi: 10.2164/jandrol.04178

Table 2.

Gene expression levels of l-arginine NO-related and steroidogenic enzymes in adult Leydig cells^*^†

Gene Code	Protein Name	Expression Level (Arbitrary Units)
A08f	iNOS	4.08 ± 1.31
C10n	eNOS	10.38 ± 1.25
F06h	nNOS	3.02 ± 0.21
C09a	Arginase 1	7.59 ± 1.33
CYP17	17α-hydroxylase	8654.86 ± 760.12
C07a	3β-HSD	2493.21 ± 599.51
C051	11-β-HSD1	380.51 ± 37.93
G27	GAPDH	179.60 ± 25.29
G43	β-Actin	1000

Gene expression levels were estimated as described in “Materials and Methods” and are presented as the ratio to the housekeeping gene β-actin, which was set at 1000. The detection level is set at twice the standard deviation of the noise at 5.4, and values represent the mean ± SEM of 4 separate determinations.

^†

NO indicates nitric oxide; iNOS, inducible NO synthase; eNOS, endothelial NO synthase; nNOS, neuronal NO synthase; HSD, hydroxysteroid dehydrogenase; and GAPDH, glyceraldehyde-3-phosphate dehydrogenase.