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. 2005 Jul 18;170(2):183–190. doi: 10.1083/jcb.200504167

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Copyright © 2005, The Rockefeller University Press

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Figure 4. — TIRFM. (A) GFP-CAPRI translocation induced by 100 μM histamine monitored by TIRFM (representative experiment for n = 4 cells). (B) GFP-RASAL translocation induced by 100 μM histamine monitored by TIRFM (representative experiment for n = 3 cells). (C) Representative experiment of GFP-CAPRI translocation in HeLa cells stimulated with 1 μM histamine (n = 3 cells). Arrows indicate mini-oscillations in one trace; similar oscillations are also detectable in other cells as shown. (D) Representative trace of sequential Fura-2 (black trace; single wavelength 380-nm excitation) and TIRF imaging of GFP-CAPRI (blue trace). Mini-oscillations correlate with cytosolic Ca²⁺ spikes.