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. Author manuscript; available in PMC: 2006 Jan 26.
Published in final edited form as: Virology. 2005 Oct 10;344(2):391–400. doi: 10.1016/j.virol.2005.09.002

Fig. 1.

Fig. 1

MΨ Puro-pA-OBD RNA capture assay. (A) Schematic representation of the plasmid transfected into 293T-based packaging cells for virus production and RNAs transcribed from this vector (not to scale). Single and double asterisks represent LTR pA and SV40 pA sites, respectively. Thin and thick lines beneath the RNAs depict RNase protection assay gag and OBD protected fragments, respectively. Ψ, packaging signal; OBD, RNA capture assay oligonucleotide binding domain. (B) Schematic overview of the RNA capture assay. Longer hooked RNAs represent OBD-containing read-through RNAs that can bind to the biotinylated oligonucleotide. Shorter RNAs represent LTR polyadenylated RNAs that lack the OBD tag. Oligonucleotide-bound dimers are captured on streptavidin-coated beads and analyzed by RNase protection assay.