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. Author manuscript; available in PMC: 2006 Jan 26.
Published in final edited form as: Biochemistry. 2004 Sep 7;43(35):11175–11186. doi: 10.1021/bi048848d

Figure 3.

Figure 3

Cellular localization of ALG-2 and Alix/AIP1. UM (A,B) and Mel290 cells (C,D) were immunostained with anti-ALG-2 antibodies followed by Texas red-conjugated secondary antibody (A,C) and with anti-Alix/AIP1 antibodies followed by FITC-conjugated secondary antibodies (B,D). Mel290 cells were immunostained with anti-ALG-2 antibodies (E) and Hoechst dye (F). Intense labeling of ALG-2 was observed throughout metaphase cells except in the region of condensed chromosomes, indicated by a white arrow. Results are shown from one of five similar experiments.