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Effect of mutations on Spx level as measured by Western blot analysis using anti-Spx antiserum. Samples were taken at T2 (2 h after the onset of the stationary phase) from cells grown in CM. (A) Lane 1, molecular mass markers; lane 2, JH642 (wild type); lane 3, ORB3834 (spx::neo); lane 4, LAB2972 (clpP::erm); lane 5, LAB2876 (clpX::spc). (B) Lane 1, molecular mass markers; lane 2, JH642; lane 3, LAB2972; lane 4, ORB3976 (clpC::tet).
