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. 2002 Jul;184(13):3664–3670. doi: 10.1128/JB.184.13.3664-3670.2002

FIG. 3.

FIG. 3.

Western blot analysis using anti-Spx antiserum. (A) Effect of culture media on Spx level as measured by Western blot analysis. Samples were taken at T2 from cells grown in DSM and in CM. Lane 1, molecular mass markers; lane 2, LAB2876 (clpX::spc) grown in DSM; lane 3, LAB2972 (clpP::erm) grown in DSM; lane 4, LAB2876 grown in CM; lane 5, LAB2972 grown in CM. (B) Cells carrying Pspac-spx were grown in CM in the presence of 1 mM IPTG. Samples were taken at T2. Lane 1, molecular mass markers; lane 2, ORB4078 (wild type); lane 3, ORB4081 (clpC::tet); lane 4, ORB4079 (clpX::spc); lane 5, ORB4080 (clpP::erm). (C) The wild type and the clpX mutant carrying Pspac-spx were grown in DSM or CM in the presence of 1 mM IPTG. Samples were taken at T2. Lane 1, molecular mass markers; lane 2, ORB4078 in DSM; lane 3, ORB4078 in CM; lane 4, ORB4079 in DSM; lane 5, ORB4079 in CM.