TABLE 1.
B. subtilis strains and plasmids used in this study
| Strain | Relevant genotype or properties | Source and/or reference |
|---|---|---|
| B. subtilis strains | ||
| JH642 | trpC2 pheA1 | J. A. Hoch |
| QPB418 | clpC::tet | 35 |
| LAB2876 | trpC2 pheA1 clpX::spc | 25 |
| LAB2972 | trpC2 pheA1 clpP::erm | 34 |
| ORB3673 | trpC2 pheA1 clpX::spc yjbFG::pPS34 | 28 |
| ORB3834 | trpC2 pheA1 yjbD::neo | 28 |
| ORB3976 | trpC2 pheA1 clpC::tet | This study |
| ORB4059 | trpC2 pheA1 yjbD-lacZ cat | This study |
| ORB4065 | trpC2 pheA1 clpX::spc yjbD-lacZ cat | This study |
| ORB4075 | trpC2 pheA1 amyE::Pspac-yjbD cat | This study |
| ORB4078 | trpC2 pheA1 yjbD::neo amyE::Pspac-yjbD | This study |
| ORB4079 | trpC2 pheA1 yjbD::neo clpX::spc amyE::Pspac-yjbD | This study |
| ORB4080 | trpC2 pheA1 yjbD::neo clpP::erm amyE::Pspac-yjbD | This study |
| ORB4081 | trpC2 pheA1 yjbD::neo clpC::tet amyE::Pspac-yjbD | This study |
| Plasmids | ||
| pDR66 | Plasmid allowing IPTG-dependent gene expression | A. Grossman, 15 |
| pLysS | Plasmid to produce T7 lysozyme | Stratagene |
| pPS34 | Integrative plasmid | P. Serror and A. L. Sonenshein, unpublished |
| pTKlac | Plasmid for construction of lacZ transcriptional fusion | 17 |
| pTYB1 | Cloning vector for IMPACT T7 system | New England BioLabs |
| pTYB2 | Cloning vector for IMPACT T7 system | New England BioLabs |
| pClpC | pTYB2 with clpC | This study |
| pClpP | pTYB1 with clpP | This study |
| pMMN464 | pPS34 carrying oppB to yjbF | This study |
| pMMN470 | pTYB4 with yjbD | 28 |
| pMMN497 | pDR66 with yjbD under control of Pspac | This study |
| pSN3 | pTYB1 with mecA | This study |
| pSN5 | pTYB1 with ypbH | This study |
| pSN16 | pTKlac carrying yjbD-lacZ | This study |