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. Author manuscript; available in PMC: 2006 Jan 26.
Published in final edited form as: Invest Ophthalmol Vis Sci. 2005 Jun;46(6):2107–2112. doi: 10.1167/iovs.05-0018

Table 1.

Primer Sequences

RT-PCR Primer Product Size (bp) Primer Sequence
MsrA forward 329 5′-AGTACCTGAGCAAGAACCCCA-3′
MsrA reverse 5′-TCACTCAGACCCCAGAAGACA-3′
MsrB1 forward 328 5′-GACGTTACACCCTCACCTT-3′
MsrB1 reverse 5′-AGCTACTTCCGCACAGATT-3′
MsrB2 forward 308 5′-CAAGGAAGCAGGAATGTATCA-3′
MsrB2 reverse 5′-ATGGTCAGTGTTTCCTTGGTTT-3′
MsrB3 forward 328 5′-CCGGGTCGTGTAGGGATAAA-3′
MsrB3 reverse 5′-TGAGCACCACACTGAGAGCA-3′
GAPDH forward 600 5′-CCACCCATGGCAAATTCCATGGCA-3′
GAPDH reverse 5′-TCTAGACGGCAGGTCAGGTCCACC-3′
siRNA siRNA Sequence
MsrA: siRNA A-8 r(CAAAGUACAAAGGAAUUUAUU) and (UAAAUUCCUUUGUACUUUGUG)
MsrB1/SelX: siRNA B1-1 r(GCGUCCGGAGCACAAUAGA)d(TT) and r(UCUAUUGUGCUCCGGACGC)d(TT)
MsrB2/CBS1: siRNA B2-1 r(GUUCUACGUCACAAGAGAA)d(TT) and r(UUCUCUUGUGACGUAGAAC)d(TG)
MsrB3: siRNA B3-1 r(GUGCCUUUGAAGGAGAAUA)d(TT) and r(UAUUCUCCUUCAAAGGCAC)d(TT)

RT-PCR primer sequences used for MsrA, -B1, -B2, and -B3 and GAPDH gene expression. SiRNA sequences used to generate MsrA, -B1, -B2, and -B3 gene silencing.