Table 1.
RT-PCR Primer | Product Size (bp) | Primer Sequence |
---|---|---|
MsrA forward | 329 | 5′-AGTACCTGAGCAAGAACCCCA-3′ |
MsrA reverse | 5′-TCACTCAGACCCCAGAAGACA-3′ | |
MsrB1 forward | 328 | 5′-GACGTTACACCCTCACCTT-3′ |
MsrB1 reverse | 5′-AGCTACTTCCGCACAGATT-3′ | |
MsrB2 forward | 308 | 5′-CAAGGAAGCAGGAATGTATCA-3′ |
MsrB2 reverse | 5′-ATGGTCAGTGTTTCCTTGGTTT-3′ | |
MsrB3 forward | 328 | 5′-CCGGGTCGTGTAGGGATAAA-3′ |
MsrB3 reverse | 5′-TGAGCACCACACTGAGAGCA-3′ | |
GAPDH forward | 600 | 5′-CCACCCATGGCAAATTCCATGGCA-3′ |
GAPDH reverse | 5′-TCTAGACGGCAGGTCAGGTCCACC-3′ | |
siRNA | siRNA Sequence | |
MsrA: siRNA A-8 | r(CAAAGUACAAAGGAAUUUAUU) and (UAAAUUCCUUUGUACUUUGUG) | |
MsrB1/SelX: siRNA B1-1 | r(GCGUCCGGAGCACAAUAGA)d(TT) and r(UCUAUUGUGCUCCGGACGC)d(TT) | |
MsrB2/CBS1: siRNA B2-1 | r(GUUCUACGUCACAAGAGAA)d(TT) and r(UUCUCUUGUGACGUAGAAC)d(TG) | |
MsrB3: siRNA B3-1 | r(GUGCCUUUGAAGGAGAAUA)d(TT) and r(UAUUCUCCUUCAAAGGCAC)d(TT) |
RT-PCR primer sequences used for MsrA, -B1, -B2, and -B3 and GAPDH gene expression. SiRNA sequences used to generate MsrA, -B1, -B2, and -B3 gene silencing.