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Western blot of total phagemid proteins. The left panel was detected with an HSA-HRP conjugate, i.e., the tag, and the right panel was detected with MAb 5G1. Lanes 1, phagemid containing the entire vmm gene fused to gene VIII; 2, helper phage R408; 3, whole-cell lysate of M. mycoides SC strain M223/90; and 4, whole-cell lysate of M. mycoides SC strain PG1. The scanned blots were processed with Corel Photo-Paint 9.0 and CorelDRAW 9.0.
