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. Author manuscript; available in PMC: 2006 Jan 26.
Published in final edited form as: Biochemistry. 2004 Nov 2;43(43):13724–13738. doi: 10.1021/bi049014y

Figure 6.

Figure 6

Effect of zinc, EDTA, and SDS on the ARE binding activity of TTP. ARE binding activity was assayed by GMSA using TTP (1 μL) purified by Ni–NTA beads and eluted with 100 mM imidazole buffer as described in the legend of Figure 4A (lane 6). The positions of TTP–probe complexes and free probes are indicated. (A) Effect of zinc and EDTA. GMSA buffers contained various concentrations of ZnCl2, EDTA, or mixtures of ZnCl2 and EDTA as indicated. (B) Effect of SDS. GMSA buffers contained various concentrations of SDS as indicated.