TABLE 3.
Complementation by membrane anchor mutants of PBP 5
| Plasmid | Mutationa | Carboxy terminusb | Primerc | Codon change | Complementationd |
|---|---|---|---|---|---|
| pPJ5 | None | FFGKIIDYIKLMFHHWFG | NA | NA | Yes |
| pAG2A | Phe2 to Ala | FAGKIIDYIKLMFHHWFG | 2 | TTC to GCC | Yes |
| pAG1A | Phe2 to Leu | FLGKIIDYIKLMFHHWFG | 1 | TTC to GAC | No |
| pAG3A | Phe2 to Asp | FDGKIIDYIKLMFHHWFG | 3 | TTC to CTG | No |
| pAG7A | Gly3 to Ala | FFAKIIDYIKLMFHHWFG | 7 | GGC to GCC | Yes |
| pAG8A | Lys4 to Ala | FFGAIIDYIKLMFHHWFG | 8 | AAA to GCA | Yes |
| pAG9A | Lys4 to His | FFGHIIDYIKLMFHHWFG | 9 | AAA to CAT | Yes |
| pAG4A | Asp7 to Ala | FFGKIIAYIKLMFHHWFG | 4 | GAT to GCG | Yes |
| pAG10A | Asp7 to Glu | FFGKIIEYIKLMFHHWFG | 10 | GAT to GAA | Yes |
| pAG6A | Ile9 to Asp | FFGKIIDYDKLMFHHWFG | 6 | ATT to GAT | No |
| pAG5A | His14 to Ala | FFGKIIDYIKLMFAHWFG | 5 | CAT to GCG | Yes |
| pAG11A | His15 to Ala | FFGKIIDYIKLMFHAWFG | 11 | CAC to GCC | No |
a
Numbering is for the last 18 amino acids of wild-type PBP 5.
b
Changed amino acids are in bold.
c
Oligonucleotide sequences of mutagenic primer pairs are available on request. NA, not applicable.
d
Complete or virtually complete restoration of normal cellular morphology to E. coli CS701-1 or CS703-1 by mutant protein.