TABLE 1.
List of strains and plasmids used in this study
| Strain or plasmid | Property | Reference or source |
|---|---|---|
| Y. enterocolitica W22703 | Human clinical isolate, wild type | 16 |
| Y. enterocolitica LC2 | Isogenic W22703 variant, Δ(sycE) | 13 |
| pDA14 | yopE promoter and full-length ORF fused to 5′ end of lacZ | This study |
| pDA36 | yopE promoter and full-length ORF fused to 5′ end of npt | 3 |
| pDA46 | yopE promoter and codons 1 to 15 fused to 5′ end of npt | 3 |
| pDA182 | yopQ promoter and full-length ORF fused to 5′ end of npt | 5 |
| pDA184 | yopQ promoter and codons 1 to 15 fused to 5′ end of npt | 5 |
| pVL9 | yopE promoter and full-length ORF fused to 5′ end of Ub gene-npt | This study |
| pVL13 | yopE promoter and codons 1 to 15 fused to 5′ end of Ub gene-npt | This study |
| pVL14 | yopE promoter and full-length ORF fused to 5′ end of UbGly3,13 gene-npt | This study |
| pVL15 | yopE promoter and codons 1 to 15 fused to 5′ end of UbGly3,13 gene-npt | This study |
| pVL16 | yopQ promoter and full-length ORF fused to 5′ end of Ub gene-npt | This study |
| pVL17 | yopQ promoter and codons 1 to 15 fused to 5′ end of Ub gene-npt | This study |
| pVL18 | yopQ promoter and full-length ORF fused to 5′ end of UbGly3,13 gene-npt | This study |
| pVL19 | yopQ promoter and codons 1 to 15 fused to 5′ end of UbGly3,13 gene-npt | This study |
| pVL20 | yopE promoter and full-length ORF fused to 5′ end of DHFR gene | This study |
| pVL21 | yopE promoter and codons 1 to 15 fused to 5′ end of DHFR gene | This study |