FIG. 1.

Genetic organization of the pig locus of X. oryzae pv. oryzae. An EcoRI restriction map of two cosmid clones, pLR9 and pLR10, is shown (E, EcoRI site). The solid black arrows represent the 14 ORFs identified in the 20,310-bp region. The direction of the arrow indicates the orientation of the ORF. The white arrows represent two ISs, ISXo3 and ISXo4. A frameshift mutation in the ISXo4 transposase is indicated by a dotted line. The filled and empty circles above the ORFs indicate the insertions that cause the Pig⁻ and Pig⁺ phenotypes, respectively. An insertion in Orf4 that affects the transport of the pigment to the outer membrane but does not affect the visible levels of pigmentation is represented by a solid rectangle. Two copies of a 145-bp XRIC sequence are shown in the orf3-ISXo4 and orf12-orf13 intergenic regions. The oppositely oriented solid triangles within these elements represent the imperfect inverted repeats.