FIG. 2.

Sequence features of putative ACP, dehydratase, and membrane transporter involved in xanthomonadin biosynthesis and localization in X. oryzae pv. oryzae. (A) Sequence alignment of a conserved domain (pfam00550) in the ACPs from X. oryzae pv. oryzae, X. fastidiosa, Haemophilus influenzae, R. leguminosarum, and Sinorhizobium meliloti. The GenBank accession numbers for these sequences are AY010120, AE003918, U32701, AF083916, and AE007237, respectively. (B) Sequence alignment of a conserved domain (pfam01377) in FabZ proteins [3-hydroxy-myristoyl-(ACP)-dehydratase] from X. oryzae pv. oryzae, E. coli, S. enterica serovar Typhimurium, and H. influenzae. The GenBank accession numbers for these sequences are AY010120, AE000127, U32786, and C37083, respectively. The conserved residues, serine (which is required for phosphopantethein attachment in ACPs) in panel A and histidine (which is known to act as the catalytic site in FabZ proteins) in panel B are shown in boldface type. The shaded and empty boxes indicate the identical and similar amino acids, respectively, in panels A and B. (C) Predicted structure of the orf4-encoded putative membrane transporter. The 12 TMDs are shown by solid cylinders spanning the cytoplasmic membrane. L1 and L2 represent the two periplasmic loops. The N- and C-terminal ends are indicated as NH₂ and COOH, respectively.