TABLE 1.
List of plasmids and strains used in this study
| Strain or plasmid | Relevant characteristic(s)a | Reference or source |
|---|---|---|
| Plasmids | ||
| pBluescript KS | Apr | Stratagene, La Jolla, Calif. |
| pMOSBlue | Apr | Amersham Pharmacia Biotech |
| pBR329 | Apr Cmr Tcr | 8 |
| pUFR034 | IncW Nmr Tra− Mob+mob(P) lacZα+ Par+cos | 9 |
| pIG102 | pLAFR3 + 25.4-kb X. campestris pv. campestris DNA that encodes pig genes | 35 |
| pLR9 | pUFR034 + 16.5-kb X. oryzae pv. oryzae insert including the pig locus | This study |
| pLR10 | pUFR034 + 21.5-kb X. oryzae pv. oryzae insert including the pig locus | This study |
| pAG13 | pBluescript KS + 5.0-kb EcoRI fragment from X. oryzae pv. oryzae pig locus | This study |
| pAG14 | pBluescript KS + 4.5-kb EcoRI fragment from X. oryzae pv. oryzae pig locus | This study |
| pAG15 | pBR329 + 12.0-kb EcoRI fragment from the pig locus of X. oryzae pv. oryzae | This study |
| pAG16 | pBluescript KS + 500-bp XhoI-SmaI fragment from orf11 | This study |
| pAG17 | pMOSBlue + 200-bp PCR-amplified fragment from orf5 | This study |
| X. oryzae pv. oryzae strains | ||
| BXO1 | Laboratory wild type, an Indian isolate | Lab collection |
| BXO43 | rif-2, derivative of BXO1 | Lab collection |
| BXO717 | pig-15::mTn5 rif-2 (a mutation in orf10) | This study |
| BXO718 | pig-16::mTn5 rif-2 (a mutation in orf6) | This study |
| BXO719 | pig-17::mTn5 rif-2 (a mutation in orf3) | This study |
| BXO720 | pig-18::mTn5 rif-2 (a mutation in orf3) | This study |
| BXO1708 | pig-55::tetA rif-2 (a mutation in orf7) | This study |
| BXO1709 | pig-56::mTn7 rif-2 (a mutation in orf4) | This study |
| BXO722 | zxx-38::mTn5 rif-2 (a mutation in orf1) | This study |
| BXO731 | zxx-10::mTn5 rif-2 (a mutation in orf1) | This study |
| BXO730 | zxx-9::mTn5 rif-2 (an insertion in the orf1-orf2 intergenic region) | This study |
| BXO727 | zxx-6::mTn5 rif-2 (a mutation in orf2) | This study |
| BXO728 | zxx-7::mTn5 rif-2 (a mutation in orf2) | This study |
| BXO729 | zxx-8::mTn5 rif-2 (a mutation in orf2) | This study |
| BXO721 | zxx-39::mTn5 rif-2 (a mutation in orf2) | This study |
| BXO1710 | zxx-40::bla rif-2 (a mutation in orf5) | This study |
| BXO724 | zxx-3::mTn5 rif-2 (a mutation in orf8) | This study |
| BXO725 | zxx-4::mTn5 rif-2 (a mutation in orf9) | This study |
| BXO1711 | zxx-35::bla rif-2 (a mutation in orf11) | This study |
| BXO1712 | zxx-36::mTn7 rif-2 (a mutation in orf12) | This study |
| BXO1713 | zxx-37::mTn7 rif-2 (a mutation in orf12) | This study |
| BXO1714 | BXO1708/pLR9-pig16::mTn5 | This study |
| BXO1715 | BXO1709/pLR9-zxx7::mTn5 | This study |
a
rif-2 indicates a mutation that confers resistance to rifampin. pig indicates mutations that cause deficiencies in either pigment production or localization. zxx indicates mutations that do not have a visible effect on pigmentation.