TABLE 2.
cAMP levels in different strains of E. coli and R. etli
| Straina | Carbon source | cAMP levels (pmol/mg of protein) |
|---|---|---|
| E. coli | ||
| W3110 (wild type) | Lactose | 318.18 ± 32.3 |
| CON1 (cya mutant) | Lactose | NDb |
| CON1(pRKACEc) | Lactose | 650.25 ± 42.8 |
| CON1(p3310) | Lactose | 125.91 ± 13.1 |
| R. etli | ||
| CE3 (wild type) | Succinate | 17.63 ± 1.9 |
| Glucose | 22.14 ± 1.7 | |
| CON31 (cyaC mutant) | Succinate | 17.15 ± 2.2 |
| Glucose | 19.99 ± 1.5 |
a
E. coli strains were grown in mm M9 supplemented with 1% lactose and 0.1% Casamino Acids. R. etli strains were grown in mm supplemented with succinate or glucose as carbon sources. The cAMP content was determined from samples of exponential growing cultures. Each value is the average of three independent experiments.
b
ND, not detected.