TABLE 1.
Plasmidsa
| Plasmid | Insert source | Insert identification |
|---|---|---|
| pCS6489 | ECOR-9 | 6-kb partial Sau3AI (ibrAB primary clone) |
| pCS6490 | ECOR-9 | 6.2-kb partial Sau3AI (ibrAB primary clone) |
| pCS7004 | pCS6490 | 2.7-kb Sau3AI-XmnI (lacks the last 34 codons of ibrA; lacks all of ibrB) |
| pCS7029 | pCS6489 | 1.4-kb SnaBI-Sau3AI (contains all of ibrB + 482-bp leader DNA) |
| pCS7038 | pCS6490 | 3.3-kb Sau3AI-BglII (contains all of ibrA; lacks the last 48 codons of ibrB) |
| pCS7052 | pCS6490 | 1.4-kb SacI-ScaI (complete ibrA ORF + 116-bp leader DNA; lacks all of ibrB) |
| pCS7067 | pCS6489 | 3-kb PvuII-BamHI (complete ibrA and ibrB ORFs + 363-bp leader DNA) |
| pCS7088 | pCS6489 | 3-kb PvuII-BamHI (complete ibrA and ibrB ORFs + 363-bp leader DNA) |
| pCS7184 | pCS7067 | 3-kb PvuII-BamHI with reading frame of ibrA ORF shifted by +4 bp at BsaHI (nt 592), causing termination after aa 235 |
| pCS7206 | pCS7067 | 3-kb PvuII-BamHI with reading frame of ibrB ORF shifted by +8 bp at MfeI (nt 279), causing altered sequence after aa 93 and termination after aa 110 |
a
All plasmids are based on the vector pOK12, except pCS7088, which is based on pUC21.