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Porphyrins in growth cultures of three different B. subtilis strains analyzed by thin-layer chromatography. Lanes 1 to 4 are reference samples, while lanes 5 to 7 are samples from different growth cultures. Lane 1, uroporphyrin III; lane 2, coproporphyrin III; lane 3, hematoporphyrin IX; lane 4, protoporphyrin IX; lane 5, B. subtilis 168; lane 6, B. subtilis 168S54A; lane 7, B. subtilis 168Q63A.
