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Catalytic properties of modified ferrochelatases compared to the wild-type enzymea
| Modification | Vmaxb |
Km (μM)
|
kcat (min−1) | |
|---|---|---|---|---|
| Zn2+ | Protoporphyrin IX | |||
| Wild type | 800 | 11 | 3.3 | 28 |
| S54A | 750 | 11 | 4.0 | 27 |
| Q63A | 49 | 12 | 5.4 | 1.7 |
The data were obtained from secondary plots of the graphs in Fig. 5.
Vmax is expressed as nanomoles of Zn-protoporphyrin IX min−1 mg of protein−1.
