FIG. 2.

Sequence alignment of LB400 BphA with variants obtained from shuffling portions of the distal segment of LB400 bphA with B-356 bphA and P6 bphA1. The shuffling strategies used to obtain these variants are described in Fig. 1. The alignment shows the amino acid positions of the three parental and variant BphAs for which at least one of the variant differs from LB400 BphA. In addition, the alignment shows the residues that correspond to regions I, II, III, and IV as designated by Mondello et al. (27).