TABLE 1.
BPDO | % Depletiona
|
|||||||
---|---|---|---|---|---|---|---|---|
2,6-CB | 3,3′CB | 4,4′CB | 2,3′,4-CB | 2,3,4′CB | 2,4,4′CB | 2,2′,5,5′CB | 2,2′,3,3′CB | |
LB400 | <10 | <10 | <10 | <10 | <10 | - | 94 | 76 |
B-356 | <10 | 35 | <10 | 96 | 63 | - | - | - |
II-4 | - | - | - | - | - | - | 84 | 47 |
II-10 | <10 | <10 | <10 | <10 | <10 | - | 56 | 24 |
III-17 | - | - | - | 73 | 60 | - | 20 | 27 |
III-34 | <10 | <10 | <10 | 79 | 88 | <10 | 63 | 23 |
II-9 | 58 | 45 | 53 | 99 | 98 | 44 | 93 | 87 |
III-37 | 36 | 58 | 43 | 96 | 98 | 29 | 85 | 65 |
III-52 | 57 | 51 | 52 | 97 | 99 | 50 | 92 | 83 |
That is, the percent depletion of each PCB congener of a mix of eight added to a culture of E. coli DH11S/pQE51[LB400-bphFGB]/pDB31[bphAE] expressing either of the BphA variants. The protocol is described in Materials and Methods. The values are averages of results from three separate experiments done in triplicate. The variance was <10% of the measured value in all cases. -, no degradation; the level of depletion of <10% was not statistically significant compared to the negative control and not reported precisely.