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. 2002 Jul;184(14):3794–3800. doi: 10.1128/JB.184.14.3794-3800.2002

TABLE 1.

PCB degradative potency of BphA variants

BPDO % Depletiona
2,6-CB 3,3′CB 4,4′CB 2,3′,4-CB 2,3,4′CB 2,4,4′CB 2,2′,5,5′CB 2,2′,3,3′CB
LB400 <10 <10 <10 <10 <10 - 94 76
B-356 <10 35 <10 96 63 - - -
II-4 - - - - - - 84 47
II-10 <10 <10 <10 <10 <10 - 56 24
III-17 - - - 73 60 - 20 27
III-34 <10 <10 <10 79 88 <10 63 23
II-9 58 45 53 99 98 44 93 87
III-37 36 58 43 96 98 29 85 65
III-52 57 51 52 97 99 50 92 83
a

That is, the percent depletion of each PCB congener of a mix of eight added to a culture of E. coli DH11S/pQE51[LB400-bphFGB]/pDB31[bphAE] expressing either of the BphA variants. The protocol is described in Materials and Methods. The values are averages of results from three separate experiments done in triplicate. The variance was <10% of the measured value in all cases. -, no degradation; the level of depletion of <10% was not statistically significant compared to the negative control and not reported precisely.