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Immunoblotting analysis of the precursor and mature forms of HycE and HybC in the wild-type strain MC4100 and in hypA and/or hybF mutant strains. Proteins (30 μg of protein from crude extracts) were separated by SDS-polyacrylamide (10%) gel electrophoresis and reacted with antibodies against HycE (A) and HybC (B). Lane 1, MC4100; lane 2, DHB-F (MC4100 ΔhybF); lane 3, SMP101; lane 4, DPABF (SMP101 ΔhybF). Panel C displays the corresponding hydrogenase activity staining.
