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. 2002 Jul;184(14):3879–3885. doi: 10.1128/JB.184.14.3879-3885.2002

FIG. 4.

FIG. 4.

Determination of the H2-dependent reduction of benzylviologen by extracts from cells grown in the presence of different nickel concentrations. Crude extracts from the wild type and the hypA ΔhybF mutant DPABF were used for this assay. Lane 1, MC4100; lanes 2 to 8, DPABF. The indicated EDTA and nickel concentrations, respectively, were present in the growth medium: lane 2, 50 μM EDTA; lane 3, 0 μM NiCl2; lane 4, 5 μM NiCl2; lane 5, 20 μM NiCl2; lane 6, 100 μM NiCl2; lane 7, 200 μM NiCl2; lane 8, 400 μM NiCl2. Please note that the ordinate is interrupted from 0.22 to 3.5 and that the maximal recovery is only about 4% in comparison to the wild type.