FIG. 5.

Immunoblotting analysis of the processing ability and hydrogenase activity of the mutant strain DPABF (SMP101 ΔhybF) grown in the presence of different concentrations of EDTA or NiCl₂ in the growth medium. Lane 1, 50 μM EDTA; lane 2, 0 μM NiCl₂; lane 3, 5 μM NiCl₂; lane 4, 20 μM NiCl₂; lane 5, 100 μM NiCl₂; lane 6, 200 μM NiCl₂; lane 7, 400 μM NiCl₂. Crude extracts (100 μg of protein) were analyzed by SDS-polyacrylamide gel electrophoresis followed by detection with antibodies raised against HycE (large subunit of hydrogenase 3) (A) and HybC (large subunit of hydrogenase 2) (B). Band X indicates nonspecific material. (C) Hydrogenase activity staining of the gel.