FIG. 2.

Introduction of ATPase activity by overexpression of F₁ genes. (A) Direct measurements of ATPase activities in E. coli LM3115 derivatives. The change in ATP concentration related to the total protein level is shown as a function of time after the addition of cellular extracts. The plasmid contents in strains BK1169(pCP44), BK1171(pCP34::atpAGD), BK1172(pCP41::atpAGD), and BK1173(pCP44::atpAGD), respectively, are indicated in the box. (B) Correlation between specific ATPase activity and specific β-galactosidase activity. (C) Correlation between specific ATPase activity with ATP, ADP, ATP+ADP pools and [ATP]/[ADP] ratios. The experimental data for the ATP, ADP, and ATP+ADP pools are fitted to linear curves shown by dotted lines, and the [ATP]/[ADP] ratio is fitted by a curve shown by a full line. Error bars indicate the standard deviations of the measurements. The data represent the average of four to six measurements.