FIG. 3.

Growth curves of E. coli BOE270 derivatives with F₁-ATPase activities. Cell density (i.e., the OD₄₅₀ value) is shown as a function of time of the cultures. The strains were grown under aerobic conditions in minimal MOPS medium (21), supplemented with thiamine (5 mg/liter); isoleucine, leucine, and valine (50 mg of each/liter); erythromycin (200 mg/liter); and limited amounts of glucose (0.4 g/liter). The plasmids and generation times of strains PJ4454(pCP44), BK1032(pCP41::atpAGD), BK1017(pCP34::atpAGD), and BK1036(pCP44::atpAGD), respectively, are indicated.