TABLE 1.

Bacterial strains and plasmids

Strain or plasmid	Relevant genotype or characteristica	Reference or source
E. coli strains
BOE270	Derived from strain MT102, which is an hsdR derivative of MC1000 [araD139 Δ(ara-leu)7679 galU galKlac-174 rpsL thi-1]	2
LM3115b	F+asnB32 thi-1 relA1 lacUV5 lacY spoT1ΔatpIBEFHAGDC750	16
PJ4454	BOE270 transformed with pCP44, Ermr	14
BK1017	BOE270 transformed with pCP34::atpAGD, Ermr	This study
BK1032	BOE270 transformed with pCP41::atpAGD, Ermr	This study
BK1036	BOE270 transformed with pCP44::atpAGD, Ermr	This study
BK1168	LM3115 transformed with pCP41, Ermr	This study
BK1169	LM3115 transformed with pCP44, Ermr	This study
BK1171	LM3115 transformed with pCP34::atpAGD, Ermr	This study
BK1172	LM3115 transformed with pCP41::atpAGD, Ermr	This study
BK1173	LM3115 transformed with pCP44::atpAGD, Ermr	This study
Plasmids
pTAC-AGD	pTTQ18 KpnI-XbaI::atpAGD KpnI-XbaI, 4.1-kb fragment, Ampr	Jensen et al.c
pAK80	Promoter probe vector, promoterless lacLM encoding the reporter enzyme β-galactosidase, Ermr	13
PCP34	PAK80 derivative carrying constitutive promoter CP34-lacLM, Ermr	14
pCP41	PAK80 derivative carrying constitutive promoter CP41-lacLM, Ermr	14
pCP44	PAK80 derivative carrying constitutive promoter CP44-lacLM, Ermr	14
pCP34::atpAGD	PCP34 BamHI-BamHI::atpAGD BamHI-BamHI, 4.5 kb, Ermr	This study
pCP41::atpAGD	PCP41 BamHI-BamHI::atpAGD BamHI-BamHI, 4.5 kb, Ermr	This study
pCP44::atpAGD	PCP44 BamHI-BamHI::atpAGD BamHI-BamHI, 4.5 kb, Ermr	This study

^aThe feature of a plasmid is indicated first by the vector ligated “::” to the insert. The restriction endonuclease used for digestion is shown. Kilobases refer to the size of an insert. Amp^r, ampicillin resistance gene; Erm^r, erythromycin resistance gene.

^bThe lacUV5 and lacY mutations have no effect on the growth of the strains, i.e., on the growth rates, growth yields, and respiration rates of the strains (15).

^cJensen et al., patent application.