TABLE 2.
Primers used in this study
| Primera | Description |
|---|---|
| F1: ACGTACGTCATATGGAAAAATTCAGCAACATTGCGAA | Amplifies 432-bp gonococcal Fur ORFb |
| R1: ACGTGGATCCTTAACGTTTGCCCTTGGCCTGACAGTC | |
| F2: CGGGATCCACAGGCGAGGATAGGGTTTGT | Amplifies 120-bp promoter fragment of gonococcal fur |
| R2: CCAAGCTTGACAGGGTAAAATACCGCTTA | |
| F3: CGGGATCCGAATTCCTATCCGATTTGCCG | Amplifies 100-bp promoter fragment of gonococcal rmp |
| R3: CCAAGCTTTTATTCCCTCATTAGATTTGTA | |
| F4: CGGGATCCCACGGTTTGCCTATGAATGG | Amplifies 100-bp promoter fragment of gonococcal tonB |
| R4: CCGGATCCGGGCATAATAATAGCAACAATTCC | |
| F5: CGGATCCGTTCCCTTTGAGCCGGGG | Amplifies 100-bp promoter fragment of gonococcal opa multigene family |
| R5: CGGATCCCGGCTCCTTATTCGGTTTGACC | |
| F6: CGGGATCCCGGTAAGGGATAAGGATGCGG | Amplifies 132-bp promoter fragment of gonococcal hemO |
| R6: CCGGATCCCCCGCTTAAAAATATAACGC | |
| F7: CGGGATCCCAACTCAAGATGTAATGGATTG | Amplifies 103-bp promoter fragment of gonococcal hmbR |
| R7: CGGGATCCCACAGATTTATTTGTACGGG | |
| F8: CGGGATCCGGCAGAGTCGGCATCCTGAATG | Amplifies 175-bp promoter fragment of hypothetical gonococcal Fe-S gene |
| R8: CCGGATCCTACTTTAAGTAATTGACCGTTGC | |
| F9: CGGGATCCGAATCGGCGGCTCACGCATTC | Amplifies 184-bp promoter fragment of gonococcal sod |
| R9: CCGGATCCCCGCCTCCATCGAATGCGGAG | |
| F10: CGGGATCCCTTTTCAGACGGCATTTTT | Amplifies 109-bp promoter fragment of gonococcal fumC |
| R10: CCAAGCTTCGTTTCTCCTTTTGAAATGT | |
| F11: CGGGATCCCGCCTACGGATGAAGAAGAGC | Amplifies 219-bp promoter fragment of gonococcal recN |
| R11: CCAAGCTTGGAATTCTACCGACAAAAGCAA | |
| F12: CCGGATCCCGTGTTGGTGGTAAGATTGAAA | Amplifies 193-bp promoter fragment of gonococcal secY |
| R12: CCAAGCTTGGCGCTTTCGTATAATTTAGC |
a
F, forward; R, reverse. Restriction sites are underlined.
b
ORF, open reading frame.