FIG. 2.

Insertion points of mini-Tn5Sm in the P. putida catabolic repression mutants isolated. The upper scheme shows the genes encoding the P. putida cyo complex (28) and the positions of the mini-Tn5Sm insertions found in mutant strains RCM4, RCM18, RCM2A, RCM2B, RCM3A, RCM6A, RCM22A, RCM117A, and RCM137A (indicated by arrows). The cyoA, cyoB, cyoC, and cyoD genes encode subunits II, I, III, and IV of the oxidase complex, respectively; cyoE encodes the heme o synthase. The bottom scheme represents the electron transport chain. NDH, NADH dehydrogenase; LDH, lactate dehydrogenase; SDH, succinate dehydrogenase; UQ, oxidized ubiquinone; UQH₂, reduced ubiquinone; cyo, cytochrome o ubiquinol oxidase.