TABLE 1.
Strains and plasmids
| Strain or plasmid | Description or relevant phenotypea | Source or reference |
|---|---|---|
| Strains | ||
| E. coli | ||
| CC118(λpir) | CC118 lysogenized with λpir phage | 25 |
| HB101 | Host for plasmid pRK600 | 5 |
| TG1 | Host for DNA manipulations | 45 |
| P. putida | ||
| KT2442 | hsdR Rif | 21 |
| PBS4 | KT2442 with PalkB::lacZ and alkS in the chromosome | 56 |
| PBS4B1 | PBS4 with an inactivated cyoB::tet allele | This work |
| PBS4C1 | PBS4 with an inactivated crc::tet allele | 57 |
| PBS4BC1 | PBS4 with an inactivated crc::xylE and cyoB::tet alleles | This work |
| RC12 | KT2442 with PalkB::kan in the chromosome | This work |
| RC12S8 | KT2442 with PalkB::kan and alkS in the chromosome | This work |
| RCM4 | RC12S8 with a mini-Tn5Sm insertion at the cyoC gene | This work |
| RCM18 | RC12S8 with a mini-Tn5Sm insertion at the cyoB gene | This work |
| RCM2A | RC12S8 with a mini-Tn5Sm insertion at the cyoB gene | This work |
| RCM3A | RC12S8 with a mini-Tn5Sm insertion at ORF2 of the cyo cluster | This work |
| RCM6A | RC12S8 with a mini-Tn5Sm insertion at the cyoA gene | This work |
| RCM22A | RC12S8 with a mini-Tn5Sm insertion at the cyoE gene | This work |
| RCM117A | RC12S8 with a mini-Tn5Sm insertion at the cyoB gene | This work |
| RCM137A | RC12S8 with a mini-Tn5Sm insertion at the cyoA gene | This work |
| RCM2B | RC12S8 with a mini-Tn5Sm insertion at the cyoE gene | This work |
| Plasmids | ||
| pALKRC1 | Apr Telr, PalkB::kan transcriptional fusion into pJMT6 | This work |
| pCRC5 | Apr; P. putida KT2442 crc gene cloned into pUC18 | 57 |
| pCRC5A | Apr; crc::xylE allele into pUC18; derives from pCRC5 | This work |
| pCRC20 | Smr; crc::xylE allele inserted at the BamHI site of pKNG101 | This work |
| pCYOB | P. putida KT2442 cyoB gene cloned into pUC18 | This work |
| pCYOBTc | cyoB::tet allele into pUC18 | This work |
| pJMT6 | Apr Telr; mini-Tn5 suicide donor plasmid | 46 |
| pKCYOBTc | cyoB::tet allele into pKNG101 | This work |
| pKNG101 | Smr; suicide vector for marker exchange mutagenesis | 29 |
| pPB7 | Apr; PalkB promoter into pUC19Ω | 56 |
| pPBK10 | Apr; PalkB::kan fusion into pUCKm | This work |
| pPBKJ1 | Apr; PalkB::kan fusion into pUJ8 | This work |
| pRK600 | Cmr Mob+ Tra+; donor of transfer functions | 30 |
| pTCS1 | Apr Tcr; alkS in mini-Tn5Tc | This work |
| pTS1 | Apr; alkS into pT7-12 | 56 |
| pUC18 | Apr; cloning vector | 55 |
| pUCKm | Apr; contains a promoterless Kmr cassette | 13 |
| pUJS1 | Apr; alkS gene cloned into pUJ8 | 56 |
| pUJ8 | Apr; vector for transcriptional fusions to lacZ | 18 |
| pUT-mini-Tn5Sm | Apr Smr; mini-Tn5 suicide donor | 18 |
| pUT-mini-Tn5Tc | Apr Tcr; mini-Tn5 suicide donor | 18 |
| pXYLE10 | Kmr; P. putida xylE gene as an excisable cassette | 48 |
a
Telr, tellurite resistant; Rifr, rifampin resistant.