FIG. 2.

E. coli two-hybrid system for detecting interactions between the C-terminal region of σ⁷⁰ and other proteins. The cartoon depicts the positions of RNA polymerase subunits β, β′, and σ⁷⁰ and the α-σ⁷⁰ chimera, which consists of the N-terminal domain of α fused to the C terminus of σ⁷⁰, at a promoter upstream of the reporter lacZ gene. The cI-bait fusion protein is located at position −62. (See text for details.)