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NodH sulfation activity on chitotriose is not inhibited by thiochitotriose methyl glycosides. Chitotriose and thiochitotriose methyl glycosides were incubated with NodH and [35S]PAPS. The reaction products were analyzed by TLC on PEI-cellulose. Lanes: 1, thiochitotriose β-methyl glycoside (β-MTG3); 2, thiochitotriose α-methyl glycoside (α-MTG3); 3, chitotriose (C3); 4, β-MTG3:C3 (1:1); 5, α-MTG3:C3 (1:1); 6, β-MTG3:C3 (10:1); 7, α-MTG3:C3 (10:1); 8, no substrate.
