FIG. 5.

Acylation of thiochitotetraose. Acylation of ³⁵S-labeled chitotetraose or thiochitooligosaccharides was assayed using permeabilized S. meliloti cells. The S. meliloti cells used were from a nodC::Tn5 mutant (TJ170/pE65) and the common nod gene deletion strain SL44. The reaction products were extracted and analyzed on Silica-Gel 60 HPTLC plates. Multiple bands near the origin may represent variable anomeric and/or acetylated substrate forms (see the text). The TJ170 extracts assayed included thiochitobiose (lane 1), thiochitotriose (lane 2), thiochitotetraose (lane 3), and chitotetraose (lane 4). The SL44 extracts assayed included thiochitobiose (lane 5), thiochitotriose (lane 6), thiochitotetraose (lane 7), and chitotetraose (lane 8). The ³⁵S-labeled Nod factor (indicated by asterisk) is also shown (lane 9).