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Organization of the S. ramocissimus tuf2 region. A restriction map of the insert from pUSRT2 is shown. Only the SacII and HgiAI restriction sites relevant to the text are shown (#). The probes used for S1 nuclease mapping of tuf2 and orfQ transcripts (asterisks, 32P-labeled 5′ ends) are shown above the restriction map. Dashed lines, nonhomologous pUC18-derived extensions.
