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. 2002 Aug;184(15):4211–4218. doi: 10.1128/JB.184.15.4211-4218.2002

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Copyright © 2002, American Society for Microbiology

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FIG. 3. — Transcription of tuf2 after kirromycin addition. (Left) Growth curve of S. ramocissimus B7 with (•) and without (○) the addition of kirromycin (5 μM) at an OD₄₅₀ of 0.6. Addition of the antibiotic to a final concentration of 25 μM resulted in a similar growth inhibition. (Right) S1 nuclease protection analysis of tuf2 transcripts in RNA isolated at the time points indicated in the left panel (C, 1, and 2). Probe, reannealed tuf2 probe; tuf2p1, transcripts initiated at tuf2p1. In lane T2, the location of the 510-nt full-length tuf2 probe can be seen. Lane M, end-labeled HpaII-digested pBR322 size markers (sizes are given in nucleotides).

FIG. 3. — Transcription of tuf2 after kirromycin addition. (Left) Growth curve of S. ramocissimus B7 with (•) and without (○) the addition of kirromycin (5 μM) at an OD₄₅₀ of 0.6. Addition of the antibiotic to a final concentration of 25 μM resulted in a similar growth inhibition. (Right) S1 nuclease protection analysis of tuf2 transcripts in RNA isolated at the time points indicated in the left panel (C, 1, and 2). Probe, reannealed tuf2 probe; tuf2p1, transcripts initiated at tuf2p1. In lane T2, the location of the 510-nt full-length tuf2 probe can be seen. Lane M, end-labeled HpaII-digested pBR322 size markers (sizes are given in nucleotides).