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Overexpression of the S. ramocissimus tuf2 and tuf3 gene products in S. coelicolor M145. S30 extracts of S. coelicolor M145 transformed with different overexpression plasmids were analyzed by Western blotting using polyclonal antibodies raised against EF-Tu1 (left) or EF-Tu3 (right). Left lane, S. coelicolor(pIJ487); middle lane, S. coelicolor(pISRT2-1); right lane, S. coelicolor(pISRT3-1).
