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Kinetic constants of wild-type, R340K, and R340A AEPT measured with d-Ala as the amino donor
| AEPTA | Km of d-Ala (mM)a | kcat (s−1) | kcat/Km of d-Ala (M−1 s−1) | kcat/Km of l-Ala (M−1 s−1) |
|---|---|---|---|---|
| Wild type | 11 ± 3 | 0.040 | 3.8 | 1,700 |
| R340K | 2.8 ± 0.5 | 0.011 | 4.0 | 38 |
| R340A | 3.7 ± 0.4 | 0.010 | 2.5 | 1.3 |
The Km of d-Ala was measured with various concentrations of d-Ala (3 to 20 mM) in the presence of a saturating concentration of 3 mM P-Ald (Km = 0.028 ± 0.005 mM).
