TABLE 2.

Plasmids employed in this study

Plasmid	Description	Phenotypea	Reference or source
S. coelicolor
pRA1	0.62-kb EcoRI/XbaI amplicon from S. coelicolor containing bldB and its promoter inserted into pSET152	Aprr	This work
pSET152	lacZα MCSbreppUCoriT φC31 int attP aac(IV)3	Aprr	2
pOJ260	reppUC AmproriT lacZα MCS	Aprr	2
pBKO	2-kb HindIII/NdeI amplicon from S. coelicolor chromosome of DNA upstream of bldB; aphI inserted at NdeI site; 1.2-kb NdeI/EcoRI amplicon of DNA downstream of bldB inserted into pOJ260	Aprr	This work
E. coli
pPCR-Script Amp	Stratagene plasmid based on pBluescript II SK(+); unique SrfI site for insertion of blunt-end amplicons	Ampr	Stratagene
pBluescriptNeo	Derived from pBluescript II SK(+); 0.9-kb AseI amplicon containing aphI inserted into pBluescript	Ampr	This work
pT18	bla ori colE1 f1 origin T18 MCS	Ampr	13
pT25	cat ori p15A T25 MCS	Chlr	13
pBB801	lacI bla ori pBR322; MRGSH6-BldB under T7 promoter/terminator expression	Ampr	R. Seyler
pT18BBH	bla ori colE1 f1 origin T18 MCS; 0.32-kb KpnI amplicon from pBB801 containing his6-bldB inserted into pT18	Ampr	This work
pT25BBH	cat ori p15A T25 MCS his6-bldB; 0.32-kb KpnI amplicon from pBB801 containing his6-bldB inserted into pT25	Chlr	This work
pT18NHB	Bla ori colE1 f1 origin T18 MCS; 0.32-kb KpnI amplicon from pBB801 containing bldB inserted into pT18	Ampr	This work
pT25NHB	cat ori p15A T25 MCS his6-bldB; 0.32-kb KpnI amplicon from pBB801 containing bldB inserted into pT25	Chlr	This work

^aAntibiotic resistance markers are apramycin (Apr^r), ampicillin (Amp^r), and chloramphenicol (Chl^r).

^bMCS, multiple cloning site.