TABLE 1.

Timeline of activities in the bacteriophage course

Day	Main activities	Key procedures	Expected outcome
Day 1	Introduction and sample collection	Instructions about sample collection and biosafety Collection of environmental samples	Samples ready for processing
Day 2	Laboratory introduction and sample preparation	Biosafety training Introduction to the equipment Introduction to bioinformatic analysis (genome assembly, genome assembly, and available tools) Sample centrifugation and filtration Preparation of enrichment cultures	Enrichment cultures prepared and incubated (overnight) Bioinformatic analysis
Day 3	Phage detection (initial screening)	Processing enrichment cultures (chloroform treatment) Preparation of double agar plates Spotting enrichment cultures Spotting filtered samples	Plates incubated for phage detection (lysis plaques expected next day) Bioinformatic analysis
Day 4	Analysis and experimental expansion	Detection of lysis zones Preparation of phage stocks Preparation of new plates for phage titration and host range assays	New plates incubated (titration and host range results expected next day) Bioinformatic analysis
Day 5	Final analysis and presentation of the results	Phage titration (PFU/mL) Host range evaluation Genomics discussion Group presentations	Interpretation of results and course conclusion