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Western immunoblot and receptor blot of total whole-cell lysate of recombinant cells with anti-FimH serum (A) and mannose-BSA (B). The apparent molecular size of the FimH-Ag43 fusion protein is indicated. (C) Coomassie brilliant blue-stained SDS-PAGE gel of wild-type and chimeric Ag43 α-modules released from the cell surface of E. coli strain HEHA16 containing plasmid pBAD/myc-HisA, pKKJ143, pKKJ135, pKKJ136, pKKJ138, or pKKJ146 (lanes 1 to 6, respectively).
