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Immunofluorescence microscopy showing surface presentation and receptor functionality of the FimH-Ag43 fusion protein on recombinant E. coli HEHA16 cells. Cells expressing plasmids pKKJ146 and pKKJ143 were reacted with primary antibodies directed against Ag43 and FimH, respectively, and FITC-labeled secondary antibody was used for detection. Additionally, receptor functionality was probed by using mannose-BSA-biotin, which was subsequently detected by FITC-conjugated streptavidin.
