TABLE 1.

Selected sequences and conserved residues of animal and bacterial HMG-CoA synthases^a

Sequence set and sourceb	Sequence (residue position)
Set 1	(95)
Animal	LEVGTETIIDKSKxVK
Bacterial	VIVATESxIDxxKAxx
Set 2	(129)
Animal	DTTNACYGGT
Bacterial	ExxxACYxAT
Set 3	(264)
Animal	MIFHxPxxK
Bacterial	xxFHxPxCK

^aCapital letters indicate conserved residues. Residues in boldface are conserved in both animal and bacterial HMG-CoA synthases. An “x” indicates a nonconserved residue. Glu95 (8), Cys129 (17), and His264 (16) have been identified as catalytic residues in the avian enzyme. For E. faecalis HMG-CoA synthase these residues are Glu79, Cys111, and His233.

^bAnimal cytosolic HMG-CoA synthases aligned are from rats, hamsters, humans, and chickens. Animal mitochondrial HMG-CoA synthases aligned are from mouse, rat, human, and pig. Aligned bacterial HMG-CoA synthase sequences are from Streptococcus pneumoniae, Streptococcus pyogenes, Enterococcus faecalis, Enterococcus faecium, Staphylococcus epidermidis, Staphylococcus haemolyticus, Staphylococcus aureus, and a Streptomyces sp.