Figure 2.

Comparative analysis of N-glycoRNAs in colorectal cancer (CRC) and normal adjacent tissue (NAT)

(A) Gel electrophoresis of total RNA and N-glycoRNA enriched using SPCgRNA from three NAT and CRC tissue samples, showing consistent glycoRNA length profiles across replicates.

(B) A heatmap illustrating the abundance of different RNA types (miRNA, rRNA, snRNA, snoRNA, tRNA, and others) in NAT and CRC, with a Venn diagram highlighting the significant overlap of 352 miRNAs shared between the two tissue types. Volcano plots depict the differential expression of glycosylated miRNAs, lncRNAs, and snoRNAs, identifying specific miRNAs (miR-21-5p, miR-19a-3p, miR-503-5p, miR-27a-3p, and miR-3458-3p) that show notable changes in CRC compared to NAT.